A SIMPLE TREATMENT OF DNA IN A LIGATION MIXTURE PRIOR TO ELECTROPORATION IMPROVES TRANSFORMATION FREQUENCY

被引：27

作者：

KOBORI, M ^{[1
]}

NOJIMA, H ^{[1
]}

机构：

[1] OSAKA UNIV,MICROBIAL DIS RES INST,DEPT MOLEC GENET,YAMADAOKA 3-1,SUITA,OSAKA 565,JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 11期

关键词：

D O I：

10.1093/nar/21.11.2782

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：2782 / 2782

页数：1

共 8 条

[1]

Dower W J, 1990, Genet Eng (N Y), V12, P275

[2] HIGH-EFFICIENCY TRANSFORMATION OF ESCHERICHIA-COLI BY HIGH-VOLTAGE ELECTROPORATION [J].

DOWER, WJ ;

MILLER, JF ;

RAGSDALE, CW .

NUCLEIC ACIDS RESEARCH, 1988, 16 (13) :6127-6145

[3] HIGH-EFFICIENCY TRANSFORMATION OF ESCHERICHIA-COLI WITH PLASMIDS [J].

INOUE, H ;

NOJIMA, H ;

OKAYAMA, H .

GENE, 1990, 96 (01) :23-28

[4] HIGH-EFFICIENCY ELECTROTRANSFORMATION OF ESCHERICHIA-COLI WITH DNA FROM LIGATION MIXTURES [J].

JACOBS, M ;

WNENDT, S ;

STAHL, U .

NUCLEIC ACIDS RESEARCH, 1990, 18 (06) :1653-1653

[5]

OKAYAMA H, 1987, METHOD ENZYMOL, V154, P3

[6] HIGH-VOLTAGE ESCHERICHIA-COLI ELECTRO-TRANSFORMATION WITH DNA FOLLOWING LIGATION [J].

WILLSON, TA ;

GOUGH, NM .

NUCLEIC ACIDS RESEARCH, 1988, 16 (24) :11820-11820

[7] HEAT INACTIVATION OF DNA-LIGASE PRIOR TO ELECTROPORATION INCREASES TRANSFORMATION EFFICIENCY [J].

YMER, S .

NUCLEIC ACIDS RESEARCH, 1991, 19 (24) :6960-6960

[8]

ZABAROVSKY ER, 1990, NUCLEIC ACIDS RES, V18, P5912

← 1 →