IMPROVEMENT OF AN IN-GEL DIGESTION PROCEDURE FOR THE MICROPREPARATION OF INTERNAL PROTEIN-FRAGMENTS FOR AMINO-ACID SEQUENCING

被引：666

作者：

HELLMAN, U

WERNSTEDT, C

GONEZ, J

HELDIN, CH

机构：

[1] Ludwig Institute for Cancer Research, S-751 24 Uppsala

来源：

ANALYTICAL BIOCHEMISTRY | 1995年 / 224卷 / 01期

关键词：

D O I：

10.1006/abio.1995.1070

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：451 / 455

页数：5

共 9 条

[1] INTERNAL AMINO-ACID SEQUENCE-ANALYSIS OF PROTEINS SEPARATED BY ONE-DIMENSIONAL OR TWO-DIMENSIONAL GEL-ELECTROPHORESIS AFTER INSITU PROTEASE DIGESTION ON NITROCELLULOSE [J].

AEBERSOLD, RH ;

LEAVITT, J ;

SAAVEDRA, RA ;

HOOD, LE ;

KENT, SBH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (20) :6970-6974

[2] TRANSFER OF PROTEINS ACROSS MEMBRANES .1. PRESENCE OF PROTEOLYTICALLY PROCESSED AND UNPROCESSED NASCENT IMMUNOGLOBULIN LIGHT-CHAINS ON MEMBRANE-BOUND RIBOSOMES OF MURINE MYELOMA [J].

BLOBEL, G ;

DOBBERSTEIN, B .

JOURNAL OF CELL BIOLOGY, 1975, 67 (03) :835-851

[3] COMBINATION OF 2-DIMENSIONAL GEL-ELECTROPHORESIS WITH MICROSEQUENCING AND AMINO-ACID-COMPOSITION ANALYSIS - IMPROVEMENT OF SPEED AND SENSITIVITY IN PROTEIN CHARACTERIZATION [J].

ECKERSKORN, C ;

LOTTSPEICH, F .

ELECTROPHORESIS, 1990, 11 (07) :554-561

[4] INTERNAL PROTEIN-SEQUENCE ANALYSIS - ENZYMATIC DIGESTION FOR LESS THAN 10 MU-G OF PROTEIN-BOUND TO POLYVINYLIDENE DIFLUORIDE OR NITROCELLULOSE MEMBRANES [J].

FERNANDEZ, J ;

DEMOTT, M ;

ATHERTON, D ;

MISCHE, SM .

ANALYTICAL BIOCHEMISTRY, 1992, 201 (02) :255-264

[5]

MATSUDAIRA P, 1987, J BIOL CHEM, V262, P10035

[6]

RASMUSSEN HH, 1991, ADV LIF SCI, P103

[7] IN-GEL DIGESTION OF PROTEINS FOR INTERNAL SEQUENCE-ANALYSIS AFTER 1-DIMENSIONAL OR 2-DIMENSIONAL GEL-ELECTROPHORESIS [J].

ROSENFELD, J ;

CAPDEVIELLE, J ;

GUILLEMOT, JC ;

FERRARA, P .

ANALYTICAL BIOCHEMISTRY, 1992, 203 (01) :173-179

[8] PROTEIN-BLOTTING ON POLYBRENE-COATED GLASS-FIBER SHEETS - A BASIS FOR ACID-HYDROLYSIS AND GAS-PHASE SEQUENCING OF PICOMOLE QUANTITIES OF PROTEIN PREVIOUSLY SEPARATED ON SODIUM DODECYL-SULFATE POLYACRYLAMIDE-GEL [J].

VANDEKERCKHOVE, J ;

BAUW, G ;

PUYPE, M ;

VANDAMME, J ;

VANMONTAGU, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 152 (01) :9-19

[9]

WARD LD, 1990, CURRENT RES PROTEIN, P179

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