RELEASE OF VIRUS-LIKE PARTICLES BY OSMOTIC SHOCK FROM A MUTANT STRAIN OF YEAST DEFICIENT IN CELL INTEGRITY

被引：5

作者：

ALVAREZ, P

SANCHEZ, M

MOLINA, M

NOMBELA, C

机构：

[1] Departamento de Microbiología II, Facultad de Farmacia, Plaza de Ramón y Cajal, Universidad Complutense, Madrid

来源：

BIOTECHNOLOGY TECHNIQUES | 1995年 / 9卷 / 06期

关键词：

D O I：

10.1007/BF00160833

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A yeast system based on a S. cerevisiae mutant strain deficient in cell integrity, was used to achieve the release of yeast expressed virus-like particles (VLPs) by simple osmotic shock. Yeast cells, grown in the presence of 1M sorbitol, lysed and released the intracellular content upon being transferred to non-osmotically stabilized medium. The release of VLPs was followed by Western blotting determination of the p1 protein.

引用

页码：441 / 444

页数：4

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