THIMEROSAL CAUSES CALCIUM OSCILLATIONS AND SENSITIZES CALCIUM-INDUCED CALCIUM RELEASE IN UNFERTILIZED HAMSTER EGGS

被引：138

作者：

SWANN, K

机构：

[1] MRC Experimental Embryology and Teratology Unit. St. George's Hospital Medical School, London, SW17 0RE, Cranmer Terrace

来源：

FEBS LETTERS | 1991年 / 278卷 / 02期

关键词：

CALCIUM ION RELEASE; HAMSTER EGG; CALCIUM ACTIVATED POTASSIUM CONDUCTANCE; SULFHYDRYL REAGENT;

D O I：

10.1016/0014-5793(91)80110-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Calcium-induced-calcium-release (CICR) was assayed in unfertilized golden hamster eggs by injecting Ca2+ and monitoring Ca2+ -dependent hyperpolarizing responses (HRs) and Ca2+ -sensitive fluo-3 fluorescence. Incubating eggs in the sulfhydryl reagent thimerosal caused [Ca2+]i oscillations as monitored by Ca2+ -dependent HRs and decreased approximately 10-fold the Ca2+ injection current required to generate an HR and cause a large intracellular Ca2+ increase. Thimerosal also enhanced the sensitivity of eggs to Ca2+ injection in a calcium-free medium. The effects of thimerosal on CICR were prevented by dithiothreitol and were not mimicked by injecting inositol 1,4,5-trisphosphate. The data suggest that thimerosal may be an alternative agent for studying CICR in caffeine-insensitive cells.

引用

页码：175 / 178

页数：4

共 29 条

[11]

JACOB R, 1990, J PHYSIOL-LONDON, V424, pP29

[12] CALCIUM OSCILLATIONS IN ELECTRICALLY NONEXCITABLE CELLS [J].

JACOB, R .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1052 (03) :427-438

[13] MODIFIED CATION ACTIVATION OF THE (NA+K)-ATPASE FOLLOWING TREATMENT WITH THIMEROSAL [J].

KAPLAN, JH ;

MONE, MD .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1985, 237 (02) :386-395

[14]

KONG SK, 1990, J PHYSIOL-LONDON, V424, pP33

[15] RHYTHMIC HYPERPOLARIZATIONS AND DEPOLARIZATION OF SYMPATHETIC-GANGLION CELLS INDUCED BY CAFFEINE [J].

KUBA, K ;

NISHI, S .

JOURNAL OF NEUROPHYSIOLOGY, 1976, 39 (03) :547-563

[16] PURIFICATION AND RECONSTITUTION OF THE CALCIUM RELEASE CHANNEL FROM SKELETAL-MUSCLE [J].

LAI, FA ;

ERICKSON, HP ;

ROUSSEAU, E ;

LIU, QY ;

MEISSNER, G .

NATURE, 1988, 331 (6154) :315-319

[17] A DELAYED ALL-OR-NONE HYPERPOLARIZATION INDUCED BY A SINGLE CA ACTION-POTENTIAL IN HAMSTER EGGS [J].

MCNIVEN, AI ;

YOSHIDA, S ;

GEORGIOU, P ;

HOUSE, CR .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1988, 412 (04) :451-453

[18]

MINTA A, 1989, J BIOL CHEM, V264, P8171

[19]

MIYAZAKI S, 1988, DEV GROWTH DIFFER, V30, P603

[20]

MIYAZAKI S, 1990, MAR JAP PHYS SOC M

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