STRUCTURAL HETEROGENEITY IN THE R173 FAMILY OF RYE-SPECIFIC REPETITIVE DNA-SEQUENCES

被引:38
作者
ROGOWSKY, PM [1 ]
LIU, JY [1 ]
MANNING, S [1 ]
TAYLOR, C [1 ]
LANGRIDGE, P [1 ]
机构
[1] UNIV ADELAIDE, WAITE AGR RES INST, CTR CEREAL BIOTECHNOL, GLEN OSMOND, SA 5064, AUSTRALIA
关键词
NUCLEOTIDE SEQUENCE; REPEATED DNA SEQUENCE; RYE (SECALE-CEREALE); TRANSPOSON;
D O I
10.1007/BF00029152
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The rye-specific R173 family of repeated DNA sequences consists of ca. 15000 individual copies per diploid rye (Secale cereale) genome and is distributed over all 7 rye chromosomes in a dispersed manner. Individual R173 elements vary in size between 3 and 6 kb, are generally not arranged as tandem repeats and are flanked by both multi-copy and single-copy sequences. DNA sequence analysis of three R173 elements (R173-1, R173-2 and R173-3) demonstrated a high degree of homology in conserved domains. The structure of R173-1 was quite different from the other two elements: long direct repeats, which represent a rye-specific repetitive sequence, were found at the ends and a 600 bp long domain was replaced by an unrelated sequence of approximately equal size. R173-2 and R173-3 were extremely similar to each other with the exception of a terminal truncation of R173-2. No open reading frames for proteins > 20 kDa were present and a database search failed to detect significant homologies to published protein sequences. Despite the transposon like genomic organisation of the R173 family, individual elements lacked sequence features frequently associated with transposons and retrotransposons. In contrast, two of the regions flanking R173 elements showed strong DNA homologies to a 850 bp long region of a proposed wheat retrotransposon and to a 300 bp long region downstream of the wheat Glu-D1 gene.
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页码:95 / 102
页数:8
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