LUMINOL CHEMILUMINESCENT DETERMINATION OF HYDROGEN-PEROXIDE AT PICOMOLE LEVELS USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH A CATION-EXCHANGE RESIN GEL COLUMN

被引：18

作者：

MIYAZAWA, T

LERTSIRI, S

FUJIMOTO, K

OKA, M

机构：

[1] Department of Applied Biological Chemistry, Tohoku University, Sendai, 981

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 667卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)89056-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The optimum conditions of high-performance liquid chromatography combined with luminol chemiluminescence detection were established for the determination of hydrogen peroxide at picomole levels using a cation-exchange gel column. The gel column with distilled water as the mobile phase allowed a good separation of H2O2 without causing any irreversible binding of H2O2 to the column surface. The detection limit and the quantification limit of H2O2 were 4 and 6-600 pmol, respectively. The suitability of the present method was verified by the determination of H2O2 present in coffee drinks.

引用

页码：99 / 104

页数：6

共 27 条

[1]

BHUNYAN KC, 1970, BIOCHIM BIOPHYS ACTA, V497, P641

[2] STEADY-STATE KINETICS OF PEROXIDASE WITH 2,2'-AZINO-DI-(3-ETHYLBENZTHIAZOLINE-6-SULPHONIC ACID) AS CHROMOGEN [J].