IDENTIFICATION AND INITIAL CHARACTERIZATION OF A PYRIMIDINE DIMER UV ENDONUCLEASE (UV ENDONUCLEASE-BETA) FROM DEINOCOCCUS-RADIODURANS - A DNA-REPAIR ENZYME THAT REQUIRES MANGANESE IONS

被引：30

作者：

EVANS, DM ^{[1
]}

MOSELEY, BEB ^{[1
]}

机构：

[1] UNIV EDINBURGH, SCH AGR, DEPT MICROBIOL, EDINBURGH EH9 3JG, MIDLOTHIAN, SCOTLAND

来源：

MUTATION RESEARCH | 1985年 / 145卷 / 03期

关键词：

D O I：

10.1016/0167-8817(85)90018-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

An endonuclease that incises lightly UV-irradiated supercoiled plasmid DNA was identified in cell-free extracts of D. radiodurans R1 wild-type. The endonuclease was absent from strains mutant in the uvsC, uvsD or uvsE genes, identifying it as UV endonuclease .beta. responsible for the initial incision step of 1 excision-repair pathway for the removal of pyrimidine dimers from D. radiodurans DNA in vivo. The enzyme was purified free from contaminating nuclease activities and was partially characterized. The enzyme has an apparent MW of 36,000, is ATP-independent, caffeine-insensitive and is inactivated by N-ethylmaleimide. It also has a novel requirement for Mn2+, distinguishing it from all other known DNA-repair enzymes.

引用

页码：119 / 128

页数：10

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