DEVELOPMENT OF AN AMPEROMETRIC ALCOHOL SENSOR BASED ON IMMOBILIZED ALCOHOL-DEHYDROGENASE AND ENTRAPPED NAD+

被引：35

作者：

MIYAMOTO, S

MURAKAMI, T

SAITO, A

KIMURA, J

机构：

[1] NEC Corporation, Central Research Laboratories, Miyamae-ku, Kawasaki, 216, 1-1

来源：

BIOSENSORS & BIOELECTRONICS | 1991年 / 6卷 / 07期

关键词：

BIOSENSOR; ALCOHOL DEHYDROGENASE; NAD+; AMPEROMETRIC BIOSENSOR; NAD+ ENTRAPMENT; BSA MEMBRANE; ALCOHOL;

D O I：

10.1016/0956-5663(91)80020-X

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

An amperometric planar alcohol sensor has been developed, which has a triple-layer enzyme-immobilized membrane. The lower layer consists of immobilized alcohol dehydrogenase (ADH), the middle layer contains entrapped mobile nicotinamide adenine dinucleotide (NAD+) molecules, and the upper layer is a denser bovine serum albumin (BSA) layer. Typical sensor response to 500 mM ethanol was 250 nA. The sensor responds sufficiently after 10 measurements and 1 month of storage at -20-degrees-C. Using this alcohol sensor, ethanol has been measured, without further addition of NAD+ or any other reactive reagent, and repeatable usage has been realized.

引用

页码：563 / 567

页数：5

共 13 条

[1] ENZYMATIC METHODS FOR THE DETERMINATION OF ETHANOL BASED ON LINEAR AND CYCLIC FLOW-INJECTION SYSTEMS [J].