AGAR MEDIUM FOR ENUMERATION OF FECAL-COLIFORMS

被引：5

作者：

CHEN, HC ^{[1
]}

WU, SD ^{[1
]}

机构：

[1] CHINA JUNIOR COLL MARINE TECHNOL,DEPT SEA PROD PROC,TAIPEI 111,TAIWAN

来源：

JOURNAL OF FOOD SCIENCE | 1992年 / 57卷 / 06期

关键词：

FECAL COLIFORMS; ENUMERATION; AGAR MEDIUM;

D O I：

10.1111/j.1365-2621.1992.tb06882.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Spread-inoculated plates of fecal-coliform agar (FCA) were pre-incubated 2 hr at 35-degrees-C, and then transferred to 44.5-degrees-C for 22 hr. Fecal coliforms on FCA produce acids and CO2 which react with bromocresol purple and calcium lactate to form yellow to yellow-green colonies surrounded by a pale yellow zone. Recovery of control or freeze-stressed Escherichia coli on FCA was about 1 log cycle lower than that with nonselective media. The accuracy for FCA to enumerate fecal coliforms from 138 typical colonies of three food samples was 98.6%; while the false positive was 1.4%. When the method was compared with the standard 3-tube MPN method for fecal coliforms, counts from two methods were not significantly different (p > 0.05).

引用

页码：1454 / 1457

页数：4

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