WESTERN-BLOT-ANALYSIS OF VIRUS-SPECIFIC ANTIBODY-RESPONSES FOR CAPRIPOX AND CONTAGIOUS PUSTULAR DERMATITIS VIRAL-INFECTIONS IN SHEEP

被引：45

作者：

CHAND, P

KITCHING, RP

BLACK, DN

机构：

[1] AFRC,INST ANIM HLTH,PIRBRIGHT LAB,WOKING GU24 0NF,SURREY,ENGLAND

[2] CCS HARYANA AGR UNIV,COLL VET SCI,DEPT VET PATHOL,HISAR 125004,HARYANA,INDIA

来源：

EPIDEMIOLOGY AND INFECTION | 1994年 / 113卷 / 02期

关键词：

D O I：

10.1017/S0950268800051803

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

This paper reports the development and evaluation of serological tests for the differentiation of antibodies in animals infected with capripox and parapox viruses. Agar-gel immunodiffusion tests using sera from sheep with naturally-acquired infections and from sheep experimentally inoculated with orf or capripox viruses showed cross reactions. Virus-specific antibody responses to structural proteins of the viruses were analysed by Western-blot analysis. This analysis readily differentiated the infections as either capripox or contagious pustular dermatitis. The antibody responses to the 32 kDa and 28 kDa proteins of capripoxvirus provided a firm basis for differention.

引用

页码：377 / 385

页数：9

共 15 条

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