CHARACTERIZATION OF GRAM-POSITIVE BROAD HOST-RANGE PLASMIDS CARRYING A THERMOPHILIC REPLICON

被引：10

作者：

DEROSSI, E ^{[1
]}

BRIGIDI, P ^{[1
]}

ROSSI, M ^{[1
]}

MATTEUZZI, D ^{[1
]}

RICCARDI, G ^{[1
]}

机构：

[1] UNIV BOLOGNA,DEPT PHARMACEUT SCI,I-40126 BOLOGNA,ITALY

来源：

RESEARCH IN MICROBIOLOGY | 1991年 / 142卷 / 04期

关键词：

THERMOPHILIA; REPLICON; PLASMID; ANTIBIOTIC RESISTANCE; BACILLUS-COAGULANS; SHUTTLE VECTORS; PBC1; ELECTROPORATION; GRAM+ BACTERIA;

D O I：

10.1016/0923-2508(91)90108-M

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The cryptic plasmid pBC1 (1.6 kb) isolated from Bacillus coagulans Zu1961 was genetically marked with the genes for chloramphenicol and ampicillin resistance (Cm(R) and Ap(R)) from the Escherichia coli plasmid pJH101. The recombinant vector obtained (pCP49, 7.0 kb) replicated and expressed Cm(R) in B. subtilis and Cm(R) and Ap(R) in E. coli. Different shuttle vectors for Gram+ bacteria were also constructed by inserting pBC1 into the Staphylococcus aureus plasmid pC194. The smallest of these, pLM6 (2.8 kb), containing essentially pBC1 and the chloramphenicol acetyl transferase gene from pC194, replicated in B. subtilis at a copy number of 60. By electroporation, these plasmids were introduced and stably maintained in B. subtilis, B. amyloliquefaciens, S. aureus, S. carnosus and Lactobacillus reuteri.

引用

页码：389 / 396

页数：8

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