MULTIPLE EPISODES OF INDUCED SECRETION OF HUMAN GROWTH-HORMONE FROM RECOMBINANT ATT-20 CELLS

被引：9

作者：

SAMBANIS, A

STEPHANOPOULOS, G

LODISH, HF

机构：

[1] Department of Chemical Engineering and Biotechnology Process Engineering Center, M.I.T., Cambridge, 02139, MA

[2] Whitehead Institute for Biomedical Research, Cambridge, 02142, MA

[3] Department of Biology and Biotechnology Process Engineering Center, M.I.T., Cambridge, 02139, MA

[4] School of Chemical Engineering, Georgia Institute of Technology, Atlanta, 30332-0100, GA

来源：

CYTOTECHNOLOGY | 1990年 / 4卷 / 02期

关键词：

AtT-20; cycling operation; human growth hormone; regulated and constitutive secretion;

D O I：

10.1007/BF00365091

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recombinant AtT-20 cells expressing human growth hormone (hGH) secreted the hormone at a constant, basal rate of 0.3-0.5 ng/105 cells-hour when exposed to medium without secretagogues. When triggered with 8 bromo-cyclic AMP, cells secreted hGH at an initial rate of 1.7 ng/105 cells-hour while intracellular hGH declined sharply. Upon extended exposure to secretagogue, secretion decreased gradually to the basal rate and intracellular hGH stabilized at a value 40% the initial. In cells switched from secretion to growth medium, the total rate of hGH accumulation intracellularly and in medium was 2.2 times that observed with cells never exposed to secretagogue; however, only a fraction of the hormone was stored intracellularly and the rest was secreted. When cells were exposed alternately to growth and secretion medium, induced cells secreted at rates at least two times higher than uninduced controls during the first five cycles. The induced response deteriorated with time, however, in parallel with outgrowth of attached cells by foci of round cells, and by the eighth cycle induced secretion did not occur. Operational modifications that may improve the performance of cycling schemes are discussed. © 1990 Kluwer Academic Publishers.

引用

页码：111 / 119

页数：9

共 13 条

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