GENETIC AND MOLECULAR CHARACTERIZATION OF EMBRYONIC MUTANTS IDENTIFIED FOLLOWING SEED TRANSFORMATION IN ARABIDOPSIS

被引：151

作者：

CASTLE, LA ^{[1
]}

ERRAMPALLI, D ^{[1
]}

ATHERTON, TL ^{[1
]}

FRANZMANN, LH ^{[1
]}

YOON, ES ^{[1
]}

MEINKE, DW ^{[1
]}

机构：

[1] OKLAHOMA STATE UNIV,DEPT BOT,STILLWATER,OK 74078

来源：

MOLECULAR & GENERAL GENETICS | 1993年 / 241卷 / 5-6期

关键词：

ARABIDOPSIS THALIANA; CHROMOSOMAL REARRANGEMENTS; EMBRYOGENESIS; PLASMID RESCUE; T-DNA INSERTIONAL MUTAGENESIS;

D O I：

10.1007/BF00279892

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Over 5000 trans,oenic families of Al Arabidopsis thaliana produced following seed transformation with Agrobacterium tumefaciens were screened for embryonic lethals, defectives, and pattern mutants. One hundred and seventy-eight mutants with a wide range of developmental abnormalities were identified. Forty-one mutants appear from genetic studies to be tagged (36% of the 115 mutants examined in detail). Mapping with visible markers demonstrated that mutant genes were randomly distributed throughout the genome. Seven mutant families appeared to contain chromosomal translocations because the mutant genes exhibited linkage to visible markers on two different chromosomes. Chromosomal rearrangements may therefore be widespread following seed transformation. DNA, gel blot hybridizations with 34 tagged mutants and three T-DNA probes revealed a wide range of insertion patterns. Models of T-DNA structure at each mutant locus were constructed to facilitate gene isolation. The value of such models was demonstrated by using plasmid rescue to clone flanking plant DNA from four tagged mutants. Further analysis of genes isolated from these insertional mutants should help to elucidate the relationship between gene function and plant embryogenesis.

引用

页码：504 / 514

页数：11

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