PROBESTIN, A NEW INHIBITOR OF AMINOPEPTIDASE-M, PRODUCED BY STREPTOMYCES-AZUREUS MH663-2F6 .1. TAXONOMY, PRODUCTION, ISOLATION, PHYSICOCHEMICAL PROPERTIES AND BIOLOGICAL-ACTIVITIES

被引：69

作者：

AOYAGI, T

YOSHIDA, S

NAKAMURA, Y

SHIGIHARA, Y

HAMADA, M

TAKEUCHI, T

机构：

[1] Institute of Microbial Chemistry, Tokyo 141, 3-14-23 Kamiosaki, Sinagawa-ku

来源：

JOURNAL OF ANTIBIOTICS | 1990年 / 43卷 / 02期

关键词：

D O I：

10.7164/antibiotics.43.143

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Probestin has been isolated as part of a program designed to find microorganism-produced inhibitors of aminopeptidase M from Streptomyces azureus MH663-2F6. It was purified by use of column chromatography of Amberlite XAD-4, silica gel, YMC-gel, Toyopearl HW-40, YMC D-ODS-5 (HPLC) and then isolated as colorless powders. Probestin is competitive with the substrate, and the inhibition constant (Ki) of it was 1.9 × 10-8M. © 1990, JAPAN ANTIBIOTICS RESEARCH ASSOCIATION. All rights reserved.

引用

页码：143 / 148

页数：6

共 10 条

[1] AMASTATIN, AN INHIBITOR OF AMINOPEPTIDASE-A, PRODUCED BY ACTINOMYCETES
AOYAGI, T
TOBE, H
KOJIMA, F
HAMADA, M
TAKEUCHI, T
UMEZAWA, H
[J]. JOURNAL OF ANTIBIOTICS, 1978, 31 (06) : 636 - 638
[2] IDENTIFICATION OF AMINOPEPTIDASE-M AS AN ENKEPHALIN-INACTIVATING ENZYME IN RAT CEREBRAL MEMBRANES
GROS, C
GIROS, B
SCHWARTZ, JC
[J]. BIOCHEMISTRY, 1985, 24 (09) : 2179 - 2185
[3] COMPOSITE EFFECTS OF ACTINONIN WHEN INHIBITING ENKEPHALIN-DEGRADING ENZYMES
HACHISU, M
HIRANUMA, T
SHIBAZAKI, Y
UOTANI, K
MURATA, S
AOYAGI, T
UMEZAWA, H
[J]. EUROPEAN JOURNAL OF PHARMACOLOGY, 1987, 137 (01) : 59 - 65
[4] IDENTIFICATION OF 2 RELATED PENTAPEPTIDES FROM BRAIN WITH POTENT OPIATE AGONIST ACTIVITY
HUGHES, J
SMITH, TW
KOSTERLITZ, HW
FOTHERGILL, LA
MORGAN, BA
MORRIS, HR
[J]. NATURE, 1975, 258 (5536) : 577 - 579
[5] SHIRLING E. B., 1966, INT J SYST BACTERIOL, V16, P313
[6] TAKEUCHI T, 1989, Patent No. 310238
[7] FOROXYMITHINE, A NEW INHIBITOR OF ANGIOTENSIN-CONVERTING ENZYME, PRODUCED BY ACTINOMYCETES
UMEZAWA, H
AOYAGI, T
OGAWA, K
OBATA, T
IINUMA, H
NAGANAWA, H
HAMADA, M
TAKEUCHI, T
[J]. JOURNAL OF ANTIBIOTICS, 1985, 38 (12) : 1813 - 1815
[8] PRODUCTION OF ACTINONIN, AN INHIBITOR OF AMINOPEPTIDASE-M, BY ACTINOMYCETES
UMEZAWA, H
AOYAGI, T
TANAKA, T
SUDA, H
OKUYAMA, A
NAGANAWA, H
HAMADA, M
TAKEUCHI, T
[J]. JOURNAL OF ANTIBIOTICS, 1985, 38 (11) : 1629 - 1630
[9] BESTATIN, AN INHIBITOR OF AMINOPEPTIDASE-B, PRODUCED BY ACTINOMYCETES
UMEZAWA, H
AOYAGI, T
SUDA, H
HAMADA, M
TAKEUCHI, T
[J]. JOURNAL OF ANTIBIOTICS, 1976, 29 (01) : 97 - 99
[10] PROBESTIN, A NEW INHIBITOR OF AMINOPEPTIDASE-M, PRODUCED BY STREPTOMYCES-AZUREUS MH663-2F6 .2. STRUCTURE DETERMINATION OF PROBESTIN
YOSHIDA, S
NAKAMURA, Y
NAGANAWA, H
AOYAGI, T
TAKEUCHI, T
[J]. JOURNAL OF ANTIBIOTICS, 1990, 43 (02) : 149 - 153

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