N-CADHERIN-ASSOCIATED PROTEINS IN CHICKEN MUSCLE

The development and functional activity of the heart depends on the regulated interaction of cardiac cells. This is in part mediated by cell-cell adhesion molecules such as N-cadherin. N-cadherin belongs to a family of Ca++-dependent, transmembrane, adhesion glycoproteins that promote cell-cell adhesion by molecular self-association extracellularly, and interact intracellularly with the cytoskeleton through highly conserved carboxy-terminal domains. In this paper we show that embryonic chicken cardiac myocytes grown in vitro display Ca++-dependent adhesion and express N-cadherin. When immunoprecipitated from detergent extracts of embryonic chicken cardiac and skeletal muscle cultures, N-cadherin associates with proteins immunologically unrelated to itself. The associated proteins are similar in molecular weight to proteins that coimmunoprecipitate with E-cadherin from human epithelial cells. We postulate that the coimmunoprecipitating proteins are involved in linking the cadherins to the cytoskeleton.