HAPLOTYPE OF MULTIPLE POLYMORPHISMS RESOLVED BY ENZYMATIC AMPLIFICATION OF SINGLE DNA-MOLECULES

被引：96

作者：

RUANO, G ^{[1
]}

KIDD, KK ^{[1
]}

STEPHENS, JC ^{[1
]}

机构：

[1] YALE UNIV,HOWARD HUGHES MED INST,HUMAN GENE MAPPING LIB,NEW HAVEN,CT 06511

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 16期

关键词：

β-globin cluster; polymerase chain reaction; single-molecule dilution;

D O I：

10.1073/pnas.87.16.6296

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have developed a reliable method for the direct resolution of haplotypes or linkage phase from individuals who are multiply heterozygous in a given genomic region. The method is based on single-molecule dilution (SMD) of genomic template and amplification via biphasic polymerase chain reaction (booster PCR). We have verified the feasibility of the SMD method for a highly polymorphic region within the β-globin cluster by analysis of triply heterozygous individuals of known haplotype. This approach should be useful in many studies in population or evolutionary genetics and in a variety of clinical settings.

引用

页码：6296 / 6300

页数：5

共 29 条

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