MONITORING OF SPATIAL EXPRESSION OF FIREFLY LUCIFERASE IN TRANSFORMED ZEBRAFISH

被引：22

作者：

MAYERHOFER, R

ARAKI, K

SZALAY, AA

机构：

[1] UNIV ALBERTA,DEPT PLANT SCI,PLANT MOLEC GENET LABS,EDMONTON,AB T6G 2P5,CANADA

[2] NATL RES INST AQUACULTURE,CELL ENGN SECT,TAMAKI,MIE 51904,JAPAN

来源：

JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE | 1995年 / 10卷 / 05期

关键词：

FIREFLY LUCIFERASE; ZEBRAFISH TRANSFORMATION; IMAGE ANALYSIS;

D O I：

10.1002/bio.1170100503

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The firefly luciferase gene attached to the cytomegalovirus promoter was transferred into zebrafish (Brachydanio rerio) by microinjection of fertilized eggs. Light emission could be monitored in vivo in eggs and throughout the early development of the fish by low-light video-image analysis. Gene expression was transient in most of the cases lasting for about 2 weeks. This gene cassette proved to be a very convenient and nondestructive transformation marker and the firefly luciferase gene appears to be a 'powerful tool for real-time imaging of tissue-specific gene expression in transgenic fish.

引用

页码：271 / 275

页数：5

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