BIOTINYLATION OF OLIGONUCLEOTIDES USING A WATER-SOLUBLE BIOTIN ESTER

被引:31
作者
BENGTSTROM, M
JUNGELLNORTAMO, A
SYVANEN, AC
机构
[1] Orion Corp, Orion Pharmaceutica, Genetic Engineering Laboratory, SF-00380, Halsinki
来源
NUCLEOSIDES & NUCLEOTIDES | 1990年 / 9卷 / 01期
关键词
D O I
10.1080/07328319008045115
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Oligonucleotides aminated at the 5’-end were biotinylated with a water soluble N-hydroxysuccinimide ester of biotin in large scale. The biotinylated oligonucleotides were purified by reversed phase HPLC. © 1990, Taylor & Francis Group, LLC. All rights reserved.
引用
收藏
页码:123 / 127
页数:5
相关论文
共 8 条