IDENTIFICATION AND MAP POSITION OF YAC CLONES COMPRISING ONE-THIRD OF THE ARABIDOPSIS GENOME

被引：55

作者：

HWANG, I

KOHCHI, T

HAUGE, BM

GOODMAN, HM

SCHMIDT, R

CNOPS, G

DEAN, C

GIBSON, S

IBA, K

LEMIEUX, B

ARONDEL, V

DANHOFF, L

SOMERVILLE, C

机构：

[1] HARVARD UNIV,SCH MED,DEPT GENET,BOSTON,MA 02115

[2] MASSACHUSETTS GEN HOSP,DEPT MOLEC BIOL,BOSTON,MA 02114

[3] JOHN INNES CTR,AFRC,INST PLANT SCI RES,CAMBRIDGE LAB,NORWICH NR4 7UJ,ENGLAND

[4] MICHIGAN STATE UNIV,US DOE,PLANT RES LAB,E LANSING,MI 48824

来源：

PLANT JOURNAL | 1991年 / 1卷 / 03期

关键词：

D O I：

10.1046/j.1365-313X.1991.t01-5-00999.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

YAC clones corresponding to 125 Arabidopsis thaliana RFLP markers have been identified. At least one YAC clone has been isolated for each of the RFLP markers tested. Based on CHEF gel analysis of 196 clones, the mean insert size of the available Arabidopsis YAC libraries is approximately 160 kb. The YACs of known genetic map location encompass about 30% of the Arabidopsis genome. The results presented here represent a first step towards assembly of an overlapping YAC library of the A. thaliana genome.

引用

页码：367 / 374

页数：8

共 11 条

[1] CLONING OF LARGE SEGMENTS OF EXOGENOUS DNA INTO YEAST BY MEANS OF ARTIFICIAL CHROMOSOME VECTORS [J].