FIREFLY LUCIFERASE SYNTHESIZES P1,P4-BIS(5'-ADENOSYL)TETRAPHOSPHATE (AP4A) AND OTHER DINUCLEOSIDE POLYPHOSPHATES+

被引：61

作者：

GURANOWSKI, A ^{[1
]}

SILLERO, MAG ^{[1
]}

SILLERO, A ^{[1
]}

机构：

[1] UNIV AUTONOMA MADRID,FAC MED,CSIC,INST INVEST BIOMED,DEPT BIOQUIM,E-28029 MADRID,SPAIN

来源：

FEBS LETTERS | 1990年 / 271卷 / 1-2期

关键词：

Diadenosine tetraphosphate; Dinucleoside polyphosphate; Firefly luciferase; Pyrophosphate; Synthetase;

D O I：

10.1016/0014-5793(90)80409-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis of P1,P4-bis(5'-adenosyl)tetraphosphate (Ap4A) has been considered, for a long time, to be catalyzed mainly by some aminoacyl-tRNA synthetases [Brevet et al. (1989) Proc. Natl. Acad. Sci. USA 86, 8275-8279]. Recently, yeast Ap4A phosphorylase, acting in reverse (Guranowski et al. (1988) Biochemistry 27, 2959-2964), was shown to synthesize Ap4A, too. In the case of the synthetases, the intermediate complex E-aminoacyl-AMP may serve as donor of AMP to ATP, yielding Ap4A. Here we demonstrate that firefly luciferase (EC 1.13.12.7) which forms the E-luciferin-AMP intermediate also synthesizes Ap4A as well as other dinucleoside polyphosphates. We suggest moreover that: other enzymes (mainly synthetases and some transferases), which catalyze the transfer of a nucleotidyl moiety, via nucleotidyl-containing intermediates and releasing PPi may produce dinucleoside polyphosphates. © 1990.

引用

页码：215 / 218

页数：4

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