REGULATION OF CL- CHANNELS BY MULTIFUNCTIONAL CAM KINASE

被引：80

作者：

NISHIMOTO, I ^{[1
]}

WAGNER, JA ^{[1
]}

SCHULMAN, H ^{[1
]}

GARDNER, P ^{[1
]}

机构：

[1] STANFORD UNIV, MED CTR, SCH MED, DEPT PHARMACOL, STANFORD, CA 94305 USA

来源：

NEURON | 1991年 / 6卷 / 04期

关键词：

D O I：

10.1016/0896-6273(91)90057-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

cAMP kinase has been shown to mediate the cAMP pathway for regulation of Cl- channels in lymphocytes, but the mediator of an alternative, Ca2+ pathway has not been identified. We show here that Ca2+ ionophore activates Cl- currents in cell-attached and whole-cell patch-clamp recordings of Jurkat T lymphocytes, but this activation is not direct. The effect of Ca2+ ionophore on whole-cell Cl- currents is inhibited by a specific peptide inhibitor of multifunctional Ca2+/calmodulin-dependent protein kinase (CaM kinase). Furthermore, Cl- channels are activated in excised patches by purified CaM kinase in a fashion that mimics the effect of Ca2+ ionophore in cell-attached recordings. These results suggest that CaM kinase mediates the Ca2+ pathway of Cl- channel activation.

引用

页码：547 / 555

页数：9

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