GENE-REGULATION DURING NEURONAL AND NONNEURONAL DIFFERENTIATION OF NTERA2 HUMAN TERATOCARCINOMA-DERIVED STEM-CELLS

被引：22

作者：

ACKERMAN, SL ^{[1
]}

KNOWLES, BB ^{[1
]}

ANDREWS, PW ^{[1
]}

机构：

[1] UNIV SHEFFIELD,DEPT BIOMED SCI,SHEFFIELD,ENGLAND

来源：

MOLECULAR BRAIN RESEARCH | 1994年 / 25卷 / 1-2期

关键词：

PRION PROTEIN GENE; AMYLOID PRECURSOR GENE; CADHERIN; SYNAPTOPHYSIC; RETINOIC ACID;

D O I：

10.1016/0169-328X(94)90293-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We constructed cDNA libraries from a clonal human teratocarcinoma-derived cell line and two retinoic acid-induced derivatives: a homogeneous population of neurons and a FACS-isolated, non-neuronal population. These libraries are large and representative of the cells from which they were derived, as determined by colony hybridization. PCR analysis indicates that the transcripts encoding P- and E-cadherin are down-regulated whereas the the prion protein (PrP) transcript is up-regulated in neurons. These cells offer a promising system for investigations of human prion infection and the cDNA libraries provide a source of neuron-specific genes.

引用

页码：157 / 162

页数：6

共 36 条

[1] RETINOIC ACID INDUCES NEURONAL DIFFERENTIATION OF A CLONED HUMAN EMBRYONAL CARCINOMA CELL-LINE INVITRO [J].