SEQUENCE-SPECIFIC PHOTOINDUCED CROSS-LINKING OF THE 2 STRANDS OF DOUBLE-HELICAL DNA BY A PSORALEN COVALENTLY LINKED TO A TRIPLE HELIX-FORMING OLIGONUCLEOTIDE

被引：229

作者：

TAKASUGI, M ^{[1
]}

GUENDOUZ, A ^{[1
]}

CHASSIGNOL, M ^{[1
]}

DECOUT, JL ^{[1
]}

LHOMME, J ^{[1
]}

THUONG, NT ^{[1
]}

HELENE, C ^{[1
]}

机构：

[1] CNRS, CTR BIOPHYS MOLEC, F-45071 ORLEANS 02, FRANCE

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 13期

关键词：

CROSS LINK; PHOTOSENSITIZATION; SEQUENCE SPECIFICITY;

D O I：

10.1073/pnas.88.13.5602

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

On the basis of the structure of DNA-psoralen bis adducts (formed by psoralen with two thymines on opposite strands), a psoralen-oligonucleotide conjugate was designed to photoinduce a cross-link between the two DNA strands at a specific sequence. Psoralen was attached via its C-5 position to a 5'-thiophosphate group of an 11-mer homopyrimidine oligonucleotide. The 11-mer binds to an 11-base-pair homopurine.homopyrimidine sequence of a DNA fragment, where it forms a triple helix. Upon near-UV-irradiation, the two strands of DNA are crosslinked at the TpA step present at the triplex-duplex junction. The reaction is specific for the homopurine.homopyrimidine DNA sequence and requires both oligonucleotide recognition of the DNA major groove and intercalation of psoralen at the triplex-duplex junction. The yield of the photoinduced cross-linking reaction is quite high (> 80%). Such psoralen-oligonucleotide conjugates are probes of sequence-specific triple-helix formation and could be used to selectively control gene expression or to induce site-directed mutations.

引用

页码：5602 / 5606

页数：5

共 28 条

[1] RELATIONSHIP BETWEEN LESIONS PHOTOINDUCED BY MONO-FUNCTIONAL AND BI-FUNCTIONAL FUROCOUMARINS IN DNA AND GENOTOXIC EFFECTS IN DIPLOID YEAST [J].