THE DEPENDENCE OF PROTON LONGITUDINAL AND TRANSVERSE RELAXATION-TIMES ON CELL-CYCLE PHASE - MOUSE MCA-TRANSFORMED 10T 1/2 TCL-15 CELLS

Attempts to determine proton NMR tongitudinal relaxation times (T1) as a function of cell‐cycle stage using cells synchronized by chemical methods have yielded conficting results (P. T. Beall, C. F. Hazlewood, and P. N. Rao), Science 192, 904 (1976); R. N. Muller et al., FEBS Lett. 114, 231 (1980); D. N. Wheatley, et al., J. Cell. Sci. 88, 13 (1987). This has raised the question whether a truc dependence of T1 on cell‐cycle phase exists. In the present study, the centrifugal elutriation technique was used to obtain relatively pure, synchronized cell populations of TCL‐15 cells (a methylcholanthrene‐transformed line of mouse 10T 5 cells) for measurement of proton NMR relaxation rates. This tecnique provides a means to procure synchronized cell populations without the use of chemical agents as in the above‐cited investigations and therefore avoid possible effects caused by the chemical agents on the NMR relaxations processes. Both T1 and the transverse relaxation time, T2, of water protons in synchronized‐cell pellets obtained in this study, exhibited a dependence on cell‐cycle phase at least for the first half of the cell cycle (G1 to S). Cells in G1 phase exhibited quantitatively higher T1 and T2 relaxation times compared to those measured for cells in mid S phase. Such changes were found to correlate with changes in water content. The distribution of cell‐cycle phases of each cell population was determined by the DNA histogram using flow cytometric methods. Possible relaxation mechanisms which may contribute to the cell‐cycle‐specific phenomena of the intracellular T1 and T2 times are discussed. © 1991 Academic Press, Inc. Copyright © 1991 Wiley‐Liss, Inc., A Wiley Company