SEQUENCING OF DOUBLE-STRANDED POLYMERASE CHAIN-REACTION PRODUCTS FOR MUTATION ANALYSIS

被引：10

作者：

ANDERSON, RD ^{[1
]}

BAO, CY ^{[1
]}

MINNICK, DT ^{[1
]}

BAXTER, J ^{[1
]}

VEIGL, ML ^{[1
]}

SEDWICK, WD ^{[1
]}

机构：

[1] CASE WESTERN RESERVE UNIV,IRELAND CANC CTR,UCRC BLDG NO 2,SUITE 200,11001 CEDAR RD,CLEVELAND,OH 44106

来源：

MUTATION RESEARCH | 1993年 / 288卷 / 01期

关键词：

DNA SEQUENCING; POLYMERASE CHAIN REACTION; DOUBLE-STRANDED DNA; MUTATION ANALYSIS;

D O I：

10.1016/0027-5107(93)90219-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

This report describes a reproducible, straightforward approach to sequencing double-stranded DNA products from the polymerase chain reaction (PCR) for analysis of mutations. The sequencing protocol is a modification of that published by Kretz (Kretz et al., 1989) and has been successful in the hands of a number of investigators working on diverse projects. Following this procedure, PCR DNA products generated from bacterial sources (including pBR322 and F' derivatives), as well as cDNA and genomic DNA from both hamster and human cell lines, have been sequenced with equal success. Close attention to the molar ratio of nucleotides to double-stranded DNA template present during the labeling reaction ensures best results.

引用

页码：181 / 185

页数：5

共 15 条

[11]

Sambrook J., 1989, MOL CLONING LAB MANU

[12] THE USE OF CHEMICAL REAGENTS IN THE DETECTION OF DNA MUTATIONS [J].

SMOOKER, PM ;

COTTON, RGH .

MUTATION RESEARCH, 1993, 288 (01) :65-77

[13] DNA-SEQUENCE ANALYSIS WITH A MODIFIED BACTERIOPHAGE-T7 DNA-POLYMERASE [J].

TABOR, S ;

RICHARDSON, CC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (14) :4767-4771

[14] RAPID LOCALIZATION OF POINT MUTATIONS IN PCR PRODUCTS BY CHEMICAL (HOT) MODIFICATION [J].

TINDALL, KR ;

WHITAKER, RA .

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 1991, 18 (04) :231-238

[15] AMPLIFICATION AND DIRECT NUCLEOTIDE SEQUENCING OF CDNA FROM THE LYSATE OF LOW NUMBERS OF DIPLOID HUMAN-CELLS [J].

YANG, JL ;

MAHER, VM ;

MCCORMICK, JJ .

GENE, 1989, 83 (02) :347-354

← 1 2 →