INVITRO TRANSFORMATION OF MESENCHYMAL CELLS DERIVED FROM EMBRYONIC MUSCLE INTO CARTILAGE IN RESPONSE TO EXTRACELLULAR-MATRIX COMPONENTS OF BONE

Implantation s.c. demineralized diaphyseal bone matrix into rats induces cartilage and bone formation in vivo. When minced skeletal muscle is cultured on hemicylinders of demineralized bone in vitro, mesenchymal cells are transformed into chondrocytes. Here, the potential of extracellular matrix components of bone to trigger cartilage differentiation in vitro was examined. Extraction of bone hemicylinders with 6 M guanidine.cntdot.HCl resulted in the absence of chondrogenesis in vitro and endochondral bone formation in vivo. Biologically inactive hemicylinders of bone were then reconstituted with the guanidine extract and also with partially purified components extracted from bone matrix and bioassayed. Reconstitution completely restored the ability to elicit chondrogenesis in vitro and endochondral bone differentiation in vivo. Reconstitution of the whole guanidine extract on Millipore filters coated with gels of tendon collagen (type I) and subsequent culture with minced skeletal muscle also resulted in cartilage induction in vitro. Extracellular matrix of bone is a repository of factors that govern local cartilage and bone differentiation.