DETECTION OF TRANSFORMING GROWTH FACTOR-ALPHA IN HUMAN URINE AND PLASMA

被引：38

作者：

KATOH, M

INAGAKI, H

KUROSAWAOHSAWA, K

KATSUURA, M

TANAKA, S

机构：

[1] NIPPON MED COLL,DEPT HYG & PUBL HLTH,BUNKYO KU,TOKYO 113,JAPAN

[2] HOECHST JAPAN LTD,PHARMA RES LABS,BIOCHEM LABS,KAWAGOE,SAITAMA 350,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 167卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)90631-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive enzyme-linked immunosorbent assay (ELISA) system for human transforming growth factor alpha (TGFα) was developed in combination with polyclonal and monoclonal antibodies. Employing this assay system, we detected TGFα like activity in normal human plasma as well as in cancer patients' urine and plasma. These TGFα were analyzed by chromatography, immunoreactivity, and EGF-TGFα receptor binding assay and found to be identical to authentic human TGFα. The presence of TGFα circulating in normal adult plasma suggests a new role of TGFα in the human body. © 1990.

引用

页码：1065 / 1072

页数：8

共 25 条

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