COMPLETE CONVERSION OF L-LACTATE INTO D-LACTATE - A GENERIC APPROACH INVOLVING ENZYMATIC CATALYSIS, ELECTROCHEMICAL OXIDATION OF NADH, AND ELECTROCHEMICAL REDUCTION OF PYRUVATE

被引：43

作者：

BIADE, AE ^{[1
]}

BOURDILLON, C ^{[1
]}

LAVAL, JM ^{[1
]}

MAIRESSE, G ^{[1
]}

MOIROUX, J ^{[1
]}

机构：

[1] UNIV PARIS 07,CNRS,UNITE 438,ELECTROCHIM MOLEC LAB,2 PL JUSSIEU,F-75251 PARIS 05,FRANCE

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1992年 / 114卷 / 03期

关键词：

D O I：

10.1021/ja00029a015

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

L-Lactate was converted into D-lactate with a yield better than 97%, the system involving stereospecific catalysis of L-lactate oxidation by the rather cheap L-lactate dehydrogenase plus electrochemical regeneration of NAD+ at the anode and electrochemical reduction of pyruvate at the cathode. Such an approach can be extended to mere deracemization or complete inversion of all types of chiral alpha-alcohol-acids provided that the dehydrogenase related to the isomer to be inverted is available. Efficiency was not limited by enzyme or coenzyme deactivations.

引用

页码：893 / 897

页数：5

共 38 条

[1] ELECTROCHEMICAL REGENERATION OF REDOX COFACTORS AND MEDIATORS - THE KEY TO BIOELECTROSYNTHESIS [J].

ALLEN, PM ;

BOWEN, WR .

TRENDS IN BIOTECHNOLOGY, 1985, 3 (06) :145-149

[2]

BARMAN TE, 1969, ENZYME HDB, V1, P47

[3] ATTEMPTS FOR THE REGENERATION OF THE COENZYME NADH BY ELECTROCHEMICAL REDUCTION AND CATALYTIC-HYDROGENATION [J].

BERGEL, A ;

DURLIAT, H ;

COMTAT, M .

JOURNAL DE CHIMIE PHYSIQUE ET DE PHYSICO-CHIMIE BIOLOGIQUE, 1987, 84 (04) :593-598

[4] ELECTROCHEMICAL REGENERATION OF NAD+ - A NEW EVALUATION OF ITS ACTUAL YIELD [J].

BONNEFOY, J ;

MOIROUX, J ;

LAVAL, JM ;

BOURDILLON, C .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS I, 1988, 84 :941-950

[5] ENZYMATIC ELECTROCATALYSIS - CONTROLLED POTENTIAL ELECTROLYSIS AND COSUBSTRATE REGENERATION WITH IMMOBILIZED ENZYME MODIFIED ELECTRODE [J].

BOURDILLON, C ;

LAVAL, JM ;

THOMAS, D .

JOURNAL OF THE ELECTROCHEMICAL SOCIETY, 1986, 133 (04) :706-711

[6] SPECTROPHOTOMETRIC INVESTIGATION OF PRODUCTS FORMED FOLLOWING THE INITIAL ONE-ELECTRON ELECTROCHEMICAL REDUCTION OF NICOTINAMIDE ADENINE-DINUCLEOTIDE (NAD+) [J].

BRESNAHAN, WT ;

ELVING, PJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 678 (02) :151-156

[7]

CHENAULT HK, 1989, BIOORG CHEM, V17, P400

[8]

Chibata I, 1976, Methods Enzymol, V44, P746

[9] A COMBINED ELECTROCHEMICAL PROCESS FOR THE REGENERATION OF CO-ENZYMES [J].

DRAKESMITH, FG ;

GIBSON, B .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1988, (22) :1493-1494

[10]

ELVING PJ, 1982, BIOELECTROCH BIOENER, V9, P365, DOI 10.1016/0302-4598(82)80026-3

← 1 2 3 4 →