SWAI, A UNIQUE RESTRICTION ENDONUCLEASE FROM STAPHYLOCOCCUS-WARNERI, WHICH RECOGNIZES 5'-ATTTAAAT-3'

被引：3

作者：

LECHNER, M

FREY, B

LAUE, F

ANTONBOTELLA, J

SMITH, CL

ANKENBAUER, W

SCHMITZ, GG

机构：

[1] BOEHRINGER MANNHEIM GMBH,BIOCHEM RES CTR,NONNENWALD 2,W-8122 PENZBERG,GERMANY

[2] UNIV CALIF BERKELEY,DEPT MOLEC & CELL BIOL,BERKELEY,CA 94720

[3] LAWRENCE BERKELEY LAB,DIV CHEM BIODYNAM,BERKELEY,CA 94720

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 09期

关键词：

D O I：

10.1093/nar/20.9.2293

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel class-11 restriction endonuclease designated Swal was purified from Staphylococcus warneri. This enzyme cleaves adenovirus 2 DNA, SV40 DNA and M13mp7 at one site each, but does not cleave lambda, PhiX174, pBR322 or pBR328 DNA. Swal recognizes the octanucleotide sequence 5'-ATTTAAAT-3', cleaving in the center of the recognition sequence creating blunt ended DNA fragments. Swal was used to digest chromosomal DNA from various microorganisms and human cells.

引用

页码：2293 / 2296

页数：4

共 19 条

[1]

BORSETTI R, UNPUB

[2]

Brown N L, 1980, Methods Enzymol, V65, P391

[3] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].