PEPSIN - NEW EXTRACTANT FOR SERUM THYROXINE BY ENZYMATIC DIGESTION OF THYROXINE BINDING-PROTEINS

被引：4

作者：

CHAU, KH ^{[1
]}

CUMMINS, LM ^{[1
]}

机构：

[1] ABBOTT LABS, ABBOTT DIAG DIV, N CHICAGO, IL 60064 USA

来源：

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM | 1976年 / 42卷 / 01期

关键词：

D O I：

10.1210/jcem-42-1-189

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The use of a pepsin solution to digest [human] serum proteins results in quantitative release of thyroxine from carrier proteins. This extraction method greatly simplifies the original Murphy and Pattee competitive protein-binding assay for serum thyroxine. The simplicity and efficiency of this extractant suggest that it may have application in radioimmunoassay and competitive protein-binding assay for nonpeptide ligands in serum.

引用

页码：189 / 192

页数：4

共 9 条

[1]

BOVEY FA, 1960, ENZYMES, V4, P63

[2] RADIOIMMUNOASSAY FOR MEASUREMENT OF THYROXINE IN UNEXTRACTED SERUM [J].