PURIFICATION OF DNA POLYMERASE-II STIMULATORY FACTOR-I, A YEAST SINGLE-STRANDED DNA-BINDING PROTEIN

被引：17

作者：

BROWN, WC ^{[1
]}

SMILEY, JK ^{[1
]}

CAMPBELL, JL ^{[1
]}

机构：

[1] CALTECH,DIV BIOL,PASADENA,CA 91125

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 02期

关键词：

DNA repair; DNA replication; Saccharomyces cerevisiae;

D O I：

10.1073/pnas.87.2.677

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Incidental to the purification of yeast DNA polymerase II was the observation that various chromatographic fractions contained activities that stimulated synthesis by this polymerase. In this paper we report the purification and initial characterization of one such factor, stimulatory factor I (SFI). SFI, which is associated with an apparent complex of three polypeptides of 66, 37, and 13.5 kDa, binds preferentially to single-stranded DNA, possibly explaining its ability to stimulate DNA polymerase II. Single-stranded DNA-binding activity is associated with the 66-kDa polypeptide.

引用

页码：677 / 681

页数：5

共 26 条

[1] ISOLATION AND PROPERTIES OF A DNA-UNWINDING PROTEIN FROM USTILAGO-MAYDIS [J].