DIFFUSION AND REACTION WITHIN POROUS PACKING MEDIA - A PHENOMENOLOGICAL MODEL

被引:17
作者
JONES, WL
DOCKERY, JD
VOGEL, CR
STURMAN, PJ
机构
[1] MONTANA STATE UNIV,CTR INTERFACIAL MICROBIAL PROC ENGN,BOZEMAN,MT 59717
[2] MONTANA STATE UNIV,DEPT MATH SCI,BOZEMAN,MT 59717
关键词
IMMOBILIZED BIOMASS; POROUS PELLET; DIFFUSION REACTION MODEL; PSEUDOMONAS-AERUGINOSA; PACKED BED;
D O I
10.1002/bit.260411005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A phenomenological model has been developed to describe biomass distribution and substrate depletion in porous diatomaceous earth (DE) pellets colonized by Pseudomonas aeruginosa. The essential features of the model are diffusion, attachment and detachment to/from pore walls of the biomass, diffusion of substrate within the pellet, and external mass transfer of both substrate and biomass in the bulk fluid of a packed bed containing the pellets. A bench-scale reactor filled with DE pellets was inoculated with P. aeruginosa and operated in plug flow without recycle using a feed containing glucose as the limiting nutrient. Steady-state effluent glucose concentrations were measured at various residence times, and biomass distribution within the pellet was measured at the lowest residence time. In the model, microorganism/substrate kinetics and mass transfer characteristics were predicted from the literature. Only the attachment and detachment parameters were treated as unknowns, and were determined by fitting biomass distribution data within the pellets to the mathematical model. The rate-limiting step in substrate conversion was determined to be internal mass transfer resistance; external mass transfer resistance and microbial kinetic limitations were found to be nearly negligible. Only the outer 5% of the pellets contributed to substrate conversion.
引用
收藏
页码:947 / 956
页数:10
相关论文
共 24 条
[1]  
ANDREWS S, 1988, HAZTECH INT C CLEVEL
[2]   ACTIVITY OF PSEUDOMONAS-AERUGINOSA IN BIOFILMS - STEADY-STATE [J].
BAKKE, R ;
TRULEAR, MG ;
ROBINSON, JA ;
CHARACKLIS, WG .
BIOTECHNOLOGY AND BIOENGINEERING, 1984, 26 (12) :1418-1424
[3]  
BIRD RB, 1960, TRANSPORT PHENOMENA, P411
[4]   BIODEGRADATION BY BACTERIA IMMOBILIZED ON CELITE PARTICLES [J].
CAUNT, P ;
CHASE, HA .
BIO-TECHNOLOGY, 1988, 6 (06) :721-725
[5]  
CHARACKLIS WG, 1990, BIOFILMS, P318
[6]  
Doedel E., 1986, AUTO SOFTWARE CONTIN
[7]   EFFECTIVENESS FACTOR CALCULATIONS FOR IMMOBILIZED ENZYME CATALYSTS [J].
FINK, DJ ;
NA, TY ;
SCHULTZ, JS .
BIOTECHNOLOGY AND BIOENGINEERING, 1973, 15 (05) :879-888
[8]  
FRIDAY DD, 1988, SAE881097 TECHN PAP
[9]  
Grady C. P., 1980, BIOL WASTEWATER TREA
[10]  
Jennings PA, 1975, THESIS U ILLINOIS UR