AN IMPROVED COLORIMETRIC METHOD TO QUANTIFY SUGAR CONTENT OF PLANT-TISSUE

被引：602

作者：

BUYSSE, J

MERCKX, R

机构：

[1] Laboratory for Soil Fertility and Soil Biology, Faculty of Agriculture, 3001 Heverlee, K.U. Leuven

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 1993年 / 44卷 / 267期

关键词：

FREE SUGARS; COLORIMETRY; ROUTINE ANALYSIS; PLANT TISSUE;

D O I：

10.1093/jxb/44.10.1627

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A modification of the phenol-sulphuric acid method to determine the bulk of soluble or insoluble sugars in plant tissue is proposed. The method is based on optimizing the phenol concentration to obtain equal colour yields for glucose, fructose and sucrose. The optimal phenol concentration is found to depend on the ethanol concentration of the measuring solution. The method is quick, easy and its costs are low, permitting its use in routine analysis.

引用

页码：1627 / 1629

页数：3

共 7 条

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