OVEREXPRESSION OF GS-ALPHA SUBUNIT IN THYROID-TUMORS BEARING A MUTATED GS-ALPHA GENE

被引：19

作者：

GORELOV, VN ^{[1
]}

DUMON, K ^{[1
]}

BARTENEVA, NS ^{[1
]}

PALM, D ^{[1
]}

ROHER, HD ^{[1
]}

GORETZKI, PE ^{[1
]}

机构：

[1] UNIV WURZBURG,DEPT BIOCHEM,W-8700 WURZBURG,GERMANY

来源：

JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY | 1995年 / 121卷 / 04期

关键词：

GS-ALPHA GENE; EXPRESSION; POINT MUTATION; IMMUNOBLOTTING; THYROID TUMORS;

D O I：

10.1007/BF01366965

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Point mutations occurring at codon 201 of the gene coding for the a subunit of the stimulatory G protein impair intrinsic GTPase activity and lead to a constitutive activation of adenylate cyclase. We have examined thyroid follicular and papillary carcinomas and follicular adenomas and found samples that bear this mutation at codon 201 of the Gsa gene. Both mutation-positive and mutation-negative tissue samples were investigated for the level of Gsa expression relative to a pool of normal thyroid tissue, using immunoblotting against two (mid-region-specific and C-end-specific) antipeptide antibodies. Using 8000 g and 100 000 g membrane fractions of homogenized tissues we have demonstrated that the Gsa proteins in normal ad neoplastic thyroid tissues are represented by three isoforms: 43 kDa, 45 kDa and 52 kDa. We have quantified and compared the amount of Gs alpha protein and find it is overexpressed in mutation-bearing tissue samples.

引用

页码：219 / 224

页数：6

共 45 条

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