HUMAN GAMMA-GAMMA-ENOLASE - 2-SITE IMMUNORADIOMETRIC ASSAY WITH A SINGLE MONOCLONAL-ANTIBODY

被引：24

作者：

FEDERSPPIEL, BSS

CRAS, P

GHEUENS, J

ANDRIES, D

LOWENTHAL, A

机构：

来源：

JOURNAL OF NEUROCHEMISTRY | 1987年 / 48卷 / 01期

关键词：

D O I：

10.1111/j.1471-4159.1987.tb13122.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A monoclonal antibody (mAb), termed BBS/NC/VI-H14 (H14), that reacts with the human enzyme .gamma..gamma.-enolase was prepared. It was directed against the .gamma.-subunit and did not cross-react with the .alpha.- or .beta.-subunit. The mAb H14 can be used for quantitative determination of .gamma..gamma.-enolase in a two-site immunoradiometric assay (two-site IRMA). It is also suitable for immunostaining formalin-fixed tissues. The specific identification of .gamma..gamma.-enolase provided by the two-site IRMA with H14 is discussed in relation to the cellular distribution of this protein.

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页码：22 / 28

页数：7

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