A SPECIFIC, SENSITIVE RADIOIMMUNOASSAY FOR PLATELET-ACTIVATING FACTOR (PAF)

被引：28

作者：

SMAL, MA

BALDO, BA

MCCASKILL, A

机构：

[1] ROYAL N SHORE HOSP,KOLLING INST MED RES,ST LEONARDS,NSW 2065,AUSTRALIA

[2] SILENUS LABS PTY LTD,HAWTHORN,VIC 3122,AUSTRALIA

[3] UNIV SYDNEY,DEPT MED,SYDNEY,NSW 2006,AUSTRALIA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 128卷 / 02期

关键词：

Anti-platelet-activating factor; Platelet-activating factor; Platelet-activating factor radioimmunoassay; Quantitation of platelet-activating factor;

D O I：

10.1016/0022-1759(90)90209-E

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A specific radioimmunoassay (RIA) has been developed for platelet-activating factor (PAF) and shown to be sensitive over the range 10-1000 pg (0.02-2 pmol). The anti-PAF antibodies showed specificity for the acetyl group at the C2 position of the PAF molecule and exhibited no significant cross-reactivity with lyso-PAF or the naturally occurring lipids including lecithin and lysolecithin. The sensitivity of the RIA was at least as good as the platelet-based assays for PAF but the RIA was simpler to perform, had a higher capacity and did not have the drawback of the inherent variability associated with the bioassays. © 1990.

引用

页码：183 / 188

页数：6

共 21 条

[1] PLATELET-ACTIVATING FACTOR AS A MEDIATOR OF ALLERGIC DISEASE [J].