IDENTIFICATION OF FRANKIA STRAINS IN NODULES BY HYBRIDIZATION OF POLYMERASE CHAIN-REACTION PRODUCTS WITH STRAIN-SPECIFIC OLIGONUCLEOTIDE PROBES

被引：44

作者：

SIMONET, P ^{[1
]}

NORMAND, P ^{[1
]}

MOIROUD, A ^{[1
]}

BARDIN, R ^{[1
]}

机构：

[1] UNIV LAVAL,CTR RECH BIOL FORESTIERE,QUEBEC CITY G1K 7P4,QUEBEC,CANADA

来源：

ARCHIVES OF MICROBIOLOGY | 1990年 / 153卷 / 03期

关键词：

Amplification; Competitivity; Frankia; Hybridization; NifH; Nodulation;

D O I：

10.1007/BF00249074

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A set of oligonucleotides has been developed to study the competitivity of two Frankia strains in the nodulation of the roots of two host plant species: Alnus glutinosa and Alnus incana. Two 20 mer-oligonucleotides, complementary to highly conserved sequences inside the nifH gene, were used as primers for the polymerase chain reaction (PCR) system in order to amplify microsymbiont DNA extracted from actinorhizae. PCR products were analyzed using two strain-specific 15-mer oligonucleotides identified in the amplified region. Hybridization data indicate that strain ACoN24d is more competitive than train ArI3 in the nodulation of both hosts. © 1990 Springer-Verlag.

引用

页码：235 / 240

页数：6

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