OFLOXACIN - SERUM AND SKIN BLISTER FLUID PHARMACOKINETICS IN THE FASTING AND NONFASTING STATE

Using an agar well diffusion assay, the concentration of ofloxacin were measured in serum and skin blister fluid after an oral dose of 300 mg given with or without a standardized meal. The apparent lag time was longer when the drug was taken with food than in the fasting stage and the serum peak concentrations occurred later; the rates of absorption being 1.72 .+-. 1.12/h (S.D.) and 3.40 .+-. 1.56/h respectively. The mean peak concentrations of each individual in serum and blister fluid were 3.8 .+-. 1.6 mg/l and 1.7 .+-. 0.5 mg/l, respectively, when the drug was taken with food, compared with 4.2 .+-. 1.9 mg/l and 2.1 .+-. 0.7 mg/l in the fasting state. The rate of penetration into blister fluid was not influenced by the intake of food. The elimination half-life was 9.0 .+-. 6.2 h in serum and 13.5 .+-. 7.8 h in blister fuid when the volunteers were fasting. The corresponding values in the non-fasting state were 6.3 .+-. 5.5 h and 10.6 .+-. 4.6 h. The ratio of the area under the concentration versus time curve (AUC0-.chi.) for blister fluid and serum was 1.20 .+-. 0.70 when ofloxacin was given without food and 1.03 .+-. 0.34 when given with the meal.