APOPTOSIS IS INDUCED BY 1-METHYL-4-PHENYLPYRIDINIUM ION (MPP(+)) IN VENTRAL MESENCEPHALIC-STRIATAL COCULTURE IN RAT

被引：143

作者：

MOCHIZUKI, H ^{[1
]}

NAKAMURA, N ^{[1
]}

NISHI, K ^{[1
]}

MIZUNO, Y ^{[1
]}

机构：

[1] TOKYO METROPOLITAN INST NEUROSCI,DEPT NEUROL,FUCHU,TOKYO 183,JAPAN

来源：

NEUROSCIENCE LETTERS | 1994年 / 170卷 / 01期

关键词：

1-METHYL-4-PHENYLPYRIDINIUM ION (MPP(+)); APOPTOSIS; VENTRAL MESENCEPHALIC-STRIATAL COCULTURE; IN SITU DNA NICK-END LABELING; PARKINSONS DISEASE;

D O I：

10.1016/0304-3940(94)90271-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Apoptosis may be an important mechanism of cell death in some experimental cell death models as well as in human neurodegenerative disorders. We report evidence to indicate that 1-methyl-4-phenylpyridinium ion (MPP(+)) induces apoptotic cell death using ventral mesencephalic and striatal co-culture; Apoptosis was defined by characteristic changes in nuclear morphology as revealed by hematoxylin-staining and DNA breaks in situ, which were demonstrated histochemically by labeling 3'-OH ends with biotinylated deoxyuridine through the action of terminal deoxynucleotidyltransferase, and DNA fragmentation, which was shown by agarose-gel electrophoresis. Apoptosis may be an important mechanism of cell death in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced parkinsonism.

引用

页码：191 / 194

页数：4

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