A PORTABLE FIBEROPTIC MONITOR FOR FLUOROMETRIC BIOASSAYS

被引：20

作者：

VO-DINH, T

GRIFFIN, GD

AMBROSE, KR

机构：

[1] Oak Ridge Natl Lab, Oak Ridge, TN,, USA, Oak Ridge Natl Lab, Oak Ridge, TN, USA

来源：

APPLIED SPECTROSCOPY | 1986年 / 40卷 / 05期

关键词：

ENZYMES - Chemical Analysis - FIBER OPTICS - Applications - WAVEGUIDES; OPTICAL; -; Applications;

D O I：

10.1366/0003702864508575

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

A simple and portable fiberoptic instrument for fluorimetric bioassays is described. The instrument is designed to be readily adaptable to commercially available biotesting wells on microplates. The microwells can also be used as interchangeable sensor heads. The utility of the fiber-optic biosensor is illustrated with a model enzyme-linked immunosorbent assay (ELISA) used to measure varying amounts of rabbit immunoglobulin G from 10 pg to 1 mu g. The detection method utilizes an enzyme-amplified immunoassay of 4-methylumbelliferone phosphate as the enzyme substrate. The sensitivity of this assay is three orders of magnitude better than that obtained with a conventional ELISA absorption spectrometric assay using a commercially available reader.

引用

页码：696 / 700

页数：5

共 11 条

[1] ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) QUANTITATIVE ASSAY OF IMMUNOGLOBULIN-G [J].

ENGVALL, E ;

PERLMANN, P .

IMMUNOCHEMISTRY, 1971, 8 (09) :871-&

[2] KINETIC BEHAVIOUR OF CALF-INTESTINAL ALKALINE PHOSPHATASE WITH 4-METHYLUMBELLIFERYL PHOSPHATE [J].

FERNLEY, HN ;

WALKER, PG .

BIOCHEMICAL JOURNAL, 1965, 97 (01) :95-&

[3]

IMASAKA T, 1979, ANAL CHEM, V51, P2032

[4]

KIANG SW, 1975, CLIN CHEM, V21, P1799

[5]

MONROE D, 1983, AM CLIN PROD REV, V2, P22

[6]

SAUNDERS GC, 1976, J CLIN MICROBIOL, V3, P604

[7]

Udenfriend S., 1969, FLUORESCENCE ASSAYS, P1

[8]

VODINH T, 1980, ANAL MEASUREMENTS IN, P47

[9]

VODINH T, 1984, P DOE OHER WORKSHOP, P45

[10]

VODINH T, 1985, Q61841 ORNL REP

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