SIMIAN HEPATITIS-A VIRUS (HAV) STRAIN AGM-27 - COMPARISON OF GENOME STRUCTURE AND GROWTH IN CELL-CULTURE WITH OTHER HAV STRAINS

被引：55

作者：

TSAREV, SA

EMERSON, SU

BALAYAN, MS

TICEHURST, J

PURCELL, RH

机构：

[1] MOSCOW POLIOMYELITIS & VIRAL ENCEPHALITIDES INST, MOSCOW, USSR

[2] WALTER REED ARMY MED CTR, DEPT VIRUS DIS, WASHINGTON, DC 20307 USA

[3] SHEMYAKIN INST BIOORGAN CHEM, MOSCOW 117871, USSR

来源：

JOURNAL OF GENERAL VIROLOGY | 1991年 / 72卷

关键词：

D O I：

10.1099/0022-1317-72-7-1677

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Fragments of cDNA representing greater than 99% of the entire genome of wild-type hepatitis A virus (HAV) strain AGM-27, isolated from an African green monkey, were obtained by the polymerase chain reaction and sequenced. Comparison with other HAV isolates revealed differences in the predicted amino acid sequence in functionally critical parts of the genome. Comparison of the biological properties of AGM-27 with those of human wild-type and cell culture-adapted HM-175 strains revealed that AGM-27 grew in cell culture significantly better than did wild-type HM-175, but not as well as cell culture-adapted HM-175. AGM-27 and cell culture-adapted HM-175 were distinguishable by their differential growth in CV-1, FRhK-4 and primary AGMK cells.

引用

页码：1677 / 1683

页数：7

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