NF-KAPPA-B SUBUNIT REGULATION IN NONTRANSFORMED CD4+ LYMPHOCYTES-T

被引：314

作者：

KANG, SM ^{[1
]}

TRAN, AC ^{[1
]}

GRILLI, M ^{[1
]}

LENARDO, MJ ^{[1
]}

机构：

[1] NIAID,IMMUNOL LAB,BETHESDA,MD 20892

来源：

SCIENCE | 1992年 / 256卷 / 5062期

关键词：

D O I：

10.1126/science.1604322

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Regulation of interleukin-2 (IL-2) gene expression by the p50 and p65 subunits of the DNA binding protein NF-kappa-B was studied in nontransformed CD4+ T lymphocyte clones. A homodimeric complex of the NF-kappa-B p50 subunit was found in resting T cells. The amount of p50-p50 complex decreased after full antigenic stimulation, whereas the amount of the NF-kappa-B p50-p65 heterodimer was increased. Increased expression of the IL-2 gene and activity of the IL-2 kappa-B DNA binding site correlated with a decrease in the p50-p50 complex. Overexpression of p50 repressed IL-2 promoter expression. The switch from p50-p50 to p50-p65 complexes depended on a protein that caused sequestration of the p50-p50 complex in the nucleus.

引用

页码：1452 / 1456

页数：5

共 66 条

[1] THE INDUCIBLE TRANSCRIPTION ACTIVATOR NF-KAPPA-B - REGULATION BY DISTINCT PROTEIN SUBUNITS [J].