DNA SEPARATION WITH FIELD-INVERSION CAPILLARY ELECTROPHORESIS

被引：20

作者：

HELLER, C

PAKLEZA, C

VIOVY, JL

机构：

[1] INST CURIE, PHYS SURFACES & INTERFACES LAB, CNRS, URA 1379, PARIS, FRANCE

[2] LAB PHYSICOCHIM STRUCT & MACROMOLEC, CNRS, URA 278, PARIS, FRANCE

[3] ECOLE SUPER PHYS & CHIM IND VILLE PARIS, PHYSICOCHIM THEORIQUE LAB, CNRS, URA 1382, PARIS, FRANCE

来源：

ELECTROPHORESIS | 1995年 / 16卷 / 08期

关键词：

DNA SEPARATION; POLYMER SOLUTION; ELECTROPHORESIS THEORY;

D O I：

10.1002/elps.11501601235

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have built an automated pulsed field capillary electrophoresis system on the basis of a commercially available device. Using entangled polymer solutions as separating matrix, we demonstrate a considerable improvement of separation of double-stranded DNA in the range of 1-50 kbp under pulsed field conditions. The influence of the main parameters, i.e. pulse frequency and electric field, is studied and the results are compared to existing electrophoresis theories.

引用

收藏

页码：1423 / 1428

页数：6

相关论文

共 29 条

[1] THE USE OF COATED AND UNCOATED CAPILLARIES FOR THE ELECTROPHORETIC SEPARATION OF DNA IN DILUTE POLYMER-SOLUTIONS [J].

BARRON, AE ;

SUNADA, WM ;

BLANCH, HW .

ELECTROPHORESIS, 1995, 16 (01) :64-74

[2] A TRANSIENT ENTANGLEMENT COUPLING MECHANISM FOR DNA SEPARATION BY CAPILLARY ELECTROPHORESIS IN ULTRADILUTE POLYMER-SOLUTIONS [J].

BARRON, AE ;

BLANCH, HW ;

SOANE, DS .

ELECTROPHORESIS, 1994, 15 (05) :597-615

[3] PULSED FIELD GEL-ELECTROPHORESIS TECHNIQUES FOR SEPARATING 1-KILOBASE TO 50-KILOBASE DNA FRAGMENTS [J].

BIRREN, BW ;

LAI, E ;

HOOD, L ;

SIMON, MI .

ANALYTICAL BIOCHEMISTRY, 1989, 177 (02) :282-286

[4] PARAMETERS OF FIELD INVERSION GEL-ELECTROPHORESIS FOR THE ANALYSIS OF POX VIRUS GENOMES [J].

BOSTOCK, CJ .

NUCLEIC ACIDS RESEARCH, 1988, 16 (10) :4239-4252

[5] ELECTROPHORETIC SEPARATIONS OF LARGE DNA-MOLECULES BY PERIODIC INVERSION OF THE ELECTRIC-FIELD [J].

CARLE, GF ;

FRANK, M ;

OLSON, MV .

SCIENCE, 1986, 232 (4746) :65-68

[6] ELECTROPHORETIC MOBILITY OF DNA IN GELS. I. NEW BIASED REPTATION THEORY INCLUDING FLUCTUATIONS [J].

DUKE, T ;

VIOVY, JL ;

SEMENOV, AN .

BIOPOLYMERS, 1994, 34 (02) :239-247

[7] MOTION OF MEGABASE DEOXYRIBONUCLEIC-ACID DURING FIELD-INVERSION GEL-ELECTROPHORESIS - INVESTIGATION BY NONLOCAL MONTE-CARLO [J].

DUKE, TAJ ;

VIOVY, JL .

JOURNAL OF CHEMICAL PHYSICS, 1992, 96 (11) :8552-8563

[8] SEPARATION OF DNA RESTRICTION FRAGMENTS BY HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS WITH LOW AND ZERO CROSS-LINKED POLYACRYLAMIDE USING CONTINUOUS AND PULSED ELECTRIC-FIELDS [J].

HEIGER, DN ;

COHEN, AS ;

KARGER, BL .

JOURNAL OF CHROMATOGRAPHY, 1990, 516 (01) :33-48

[9] ELECTROPHORETIC MOBILITY OF DNA IN GELS .2. SYSTEMATIC EXPERIMENTAL-STUDY IN AGAROSE GELS [J].

HELLER, C ;

DUKE, T ;

VIOVY, JL .

BIOPOLYMERS, 1994, 34 (02) :249-259

[10] A SYSTEMATIC STUDY OF FIELD INVERSION GEL-ELECTROPHORESIS [J].

HELLER, C ;

POHL, FM .

NUCLEIC ACIDS RESEARCH, 1989, 17 (15) :5989-6003