ALVEOLAR SURFACTANT SUBFRACTIONS DIFFER IN THEIR LIPID-COMPOSITION

Alveolar surfactant consists of subfractions which are generated during normal lung function, Although subfractions obtained by differential centrifugation of lung lavage differ in structure, function and protein content, the phospholipid-pattern shows only minor differences, To correlate possible differences in composition between subfractions to their functional properties we did a more detailed analysis of lipid pattern, Subfractions of lung lavages from Wistar rats were obtained by differential centrifugation, lipid classes were separated by thin layer chromatography (TLC), Fatty acids and plasmalogens were determined as methylester and dimethylacetals by gas chromatography, respectively, Cholesterol and vitamin E were determined enzymatically and by HPLC, respectively, The patterns of fatty acids of total lipids and of the molecular species of phosphatidylcholine and phosphatidylethanolamine were very similar among the subfractions. The distribution of individual lavage lipids varied considerably. Three types of subfractions can be distinguished: The two dense subfractions (1000 g and 60,000 g) contain 70-88% of total phospholipids, dipalmitoylphosphatidylcholine, polyunsaturated phospholipids and polyunsaturated fatty acids present in lung lavage, The less dense subfraction (100,000 g) contains 44-60% of total cholesterol, choline plasmalogen, ethanalamine plasmalogen and vitamin E, The 100,000 g supernatant contains 40-50% of total tri-, diacylglycerols and free fatty acids, Our results support the concept that the 1000 g subfraction contains freshly secreted surfactant, The 60,000 g subfraction likely contains the monolayer and freshly secreted surfactant, The 100,000 g pellet probably contains material ''squeezed out'' from the monolayer at expiration, Most likely, the supernatant contains material destined for removal from the airspace.