SITE-DIRECTED MUTAGENESIS OF AZOTOBACTER-VINELANDII FERREDOXIN-I - [FE-S] CLUSTER-DRIVEN PROTEIN REARRANGEMENT

被引：105

作者：

MARTIN, AE

BURGESS, BK

STOUT, CD

CASH, VL

DEAN, DR

JENSEN, GM

STEPHENS, PJ

机构：

[1] UNIV CALIF IRVINE,DEPT MOLEC BIOL & BIOCHEM,IRVINE,CA 92717

[2] SCRIPPS CLIN & RES FDN,RES INST,DEPT MOLEC BIOL,LA JOLLA,CA 92037

[3] VIRGINIA POLYTECH INST & STATE UNIV,DEPT ANAEROB MICROBIOL,BLACKSBURG,VA 24061

[4] UNIV SO CALIF,DEPT CHEM,LOS ANGELES,CA 90089

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 02期

关键词：

D O I：

10.1073/pnas.87.2.598

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Azotobacter vinelandii ferredoxin I is a small protein that contains one [4Fe-4S] cluster and one [3Fe-4S] cluster. Recently the x-ray crystal structure has been redetermined and the fdxA gene, which encodes the protein, has been cloned and sequenced. Here we report the site-directed mutation of Cys-20, which is a ligand of the [4Fe-4S] cluster in the native protein, to alanine and the characterization of the protein product by x-ray crystallographic and spectroscopic methods. The data show that the mutant protein again contains one [4Fe-4S] cluster and one [3Fe-4S] cluster. The new [4Fe-4S] cluster obtains its fourth ligand from Cys-24, a free cysteine in the native structure. The formation of this [4Fe-4S] cluster drives rearrangement of the protein structure.

引用

页码：598 / 602

页数：5

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