EFFICIENT LARGE-SCALE PURIFICATION OF NONHISTONE CHROMOSOMAL-PROTEINS HMG1 AND HMG2 BY USING POLYBUFFER-EXCHANGER PBE94

被引：46

作者：

ADACHI, Y

MIZUNO, S

YOSHIDA, M

机构：

[1] TOHOKU UNIV,DEPT AGR CHEM,SENDAI,MIYAGI 980,JAPAN

[2] SCI UNIV TOKYO,DEPT BIOL SCI & TECHNOL,NODA,CHIBA 278,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 530卷 / 01期

关键词：

D O I：

10.1016/S0378-4347(00)82300-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the efficient and practical large-scale purification of high-mobility group (HMG) non-histone chromosomal proteins, HMG1 and HMG2, from porcine thymus applying Polybuffer-exchanger PBE94 gel as anion-exchanger has been developed. This method affords higher resolution, purity and yield, than the conventional procedure of CM-Sephadex C-25 ion-exchange column chromatography. Furthermore, use of Polybuffer-exchanger PBE94 column chromatography led to direct preparation of HMG1 and HMG2 from loosely bound non-histone chromosomal protein fraction of chromatin without prefractional precipitation with trichloroacetic acid or prior extraction with perchloric acid. Thus, the application of PBE94 gel as an anion-exchanger to the subfractionation of other kinds of homologous protein is possible. © 1990.

引用

页码：39 / 46

页数：8

共 18 条

[1]

BONNE C, 1982, J BIOL CHEM, V257, P2722

[2] IMPROVED LARGE-SCALE FRACTIONATION OF HIGH MOBILITY GROUP NON-HISTONE CHROMATIN PROTEINS [J].