PURIFICATION OF RECOMBINANT HUMAN INTERFERON-BETA BY IMMOBILIZED ANTISENSE PEPTIDES

被引：28

作者：

SCAPOL, L ^{[1
]}

RAPPUOLI, P ^{[1
]}

VISCOMI, GC ^{[1
]}

机构：

[1] SCLAVO SPA,I-53100 SIENNA,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 600卷 / 02期

关键词：

D O I：

10.1016/0021-9673(92)85553-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Synthetic antisense peptides encoded in the antisense strands of DNA corresponding to the 1-14, 42-54 and 103-115 fragments of the human interferon-beta sequence were applied in the purification of recombinant human interferon-beta from a mammalian cell culture. The protein fragments were selected on the basis of their computer-predicted exposure on the surface of the protein. The antisense peptides were synthetized by the solid-phase method directly on the resin used as the stationary phase in affinity chromatography. All the tested antisense peptides showed a selective affinity for human interferon-beta, permitting a ten-fold purification of the protein.

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页码：235 / 242

页数：8

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