INCREASED DT-DIAPHORASE ACTIVITY IN TRANSFORMED AND TUMORIGENIC PANCREATIC ACINAR-CELLS

被引：4

作者：

HAMMONS, GJ ^{[1
]}

WARREN, GJ ^{[1
]}

BLANN, E ^{[1
]}

NICHOLS, J ^{[1
]}

LYNCOOK, BD ^{[1
]}

机构：

[1] NATL CTR TOXICOL RES,DIV NUTR TOXICOL,JEFFERSON,AR 72079

来源：

CANCER LETTERS | 1995年 / 96卷 / 01期

关键词：

DT-DIAPHORASE; PANCREATIC ACINAR CELLS; TRANSFORMATION;

D O I：

10.1016/0304-3835(95)03911-F

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Pancreatic acinar cells from rats treated in vitro with 5-azacytidine and/or transfected with an activated c-H-ras demonstrated transformation and tumorigenic phenotypes. DT-diaphorase (NAD(P)H:quinone oxidoreductase) activity was determined in these non-tumorigenic (3AP) and tumorigenic cells (T3AP and T5AM). T5AM cells were those treated with 5-azacytidine and further treated with N'-methyl-N'-nitro-nitrosoguanidine. Higher levels of enzyme activity were found in transformed cells when compared to that in control cells (>15-fold, 3AP cells; >40-fold, T3AP cells; >20-fold T5AM cells). In contrast, NADPH-cytochrome c reductase activity was decreased in transformed cells (>10-fold, 3AP cells; >20-fold, T3AP cells; >10-fold, T5AM cells). These studies demonstrated that pancreatic acinar cells are capable of undergoing alterations in enzyme activity patterns when transformed and that DT-diaphorase may be a good marker for malignant transformation.

引用

页码：9 / 14

页数：6

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