INITIATION OF V(D)J RECOMBINATION IN A CELL-FREE SYSTEM

被引：256

作者：

VANGENT, DC

MCBLANE, JF

RAMSDEN, DA

SADOFSKY, MJ

HESSE, JE

GELLERT, M

机构：

[1] Laboratory of Molecular Biology National Institute of Diabetes and Digestive, Kidney Diseases National Institutes of Health Bethesda

来源：

CELL | 1995年 / 81卷 / 06期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0092-8674(95)90012-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cells performing V(D)J recombination make specific cuts in DNA at recombination signal sequences. Here, we show that nuclear extracts of pre-a cell lines carry out this specific cleavage. The products of cleavage are the same as found previously in thymocytes: full-length, blunt, 5'-phosphorylated signal ends, and covalently sealed (hairpin) coding ends. A complete signal sequence is required. Recombinant RAG1 protein greatly increases activity and complements an inactive extract from a RAG1 (-/-) pre-a cell line. When the extracts are fractionated, cleavage activity correlates with the presence of RAG2 protein. These results suggest that RAG1 and RAG2 are components of the V(D)J recombinase.

引用

页码：925 / 934

页数：10

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