DIFFERENTIAL EXPRESSION OF THE 5 C4-RELATED GENES OF H-2W7 MICE

Mice bearing the H-2w7 haplotype have five C4-related genes, one C4, one Slp, and three C4/Slp hybrid genes. The expression of these five genes in the liver of H-2w7 mice was estimated at the steady state level of their respective mRNA. We have amplified by the polymerase chain reaction (PCR) three regions of the C4/Slp mRNA where some of these five genes show nucleotide substitution. A relative amount of each gene product was estimated by single-strand conformation polymorphism (SSCP) analysis or by direct counting of the number of respective clones after subcloning into a plasmid vector. A steady state level of the C4 mRNA was most abundant among C4-related gene transcripts. The hybrid 1 and 3 genes were expressed at a similar level which is about 1/2-1/3 of the C4 level. The hybrid 2 gene was expressed at about 1/5 of the hybrid 1 or 3 level. Neither male nor female H-2w7 mice expressed the Slp gene. These results showed that the expression of the five C4-related genes of H-2w7 mice is differentially regulated in spite of the close similarity in the nucleotide sequences in both the 5' flanking and coding regions of these genes.